› 3ust

X-ray diffraction
2.1Å resolution

Structure of BmNPV ORF075 (p33)

Released:
DOI: 10.2210/pdb3ust/pdb
PDB entry 3ust coloured by chain, front view.
PDB entry 3ust coloured by chain, side view.
PDB entry 3ust coloured by chain, top view.
Source organism: Bombyx mori nucleopolyhedrovirus
Primary publication:
Crystal structure of Bombyx mori nucleopolyhedrovirus ORF75 reveals a pseudo-dimer of thiol oxidase domains with a putative substrate-binding pocket.
Hou Y, Xia Q, Yuan YA
J Gen Virol 93 2142-2151 (2012)
PMID: 22764321

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131652 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p33 Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 32.61 KDa
Source organism: Bombyx mori nucleopolyhedrovirus
Expression system: Escherichia coli
UniProt:
  • Canonical: O92452 (Residues: 1-259; Coverage: 100%)
Gene names: Bm(Br)Orf-80, Bmnpvcubicgp078, Bmnpvguangxigp078, Bmnpvindiagp077, Bmnpvzhejianggp078, Orf_75, bmnpvc2gp074, p33
Sequence domains: Baculovirus P33

Ligands and Environments

1 bound ligand:
Ligand FAD 1 x FAD
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U, RIGAKU MICROMAX-007 HF
Spacegroup: C2221
Unit cell:
a: 54.52Å b: 80.563Å c: 130.853Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.241
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

P33 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus is a functional homolog of AcP33.
Kuang et al. (2016)
3 more

1 mention without citation

Three Conserved Regions in Baculovirus Sulfhydryl Oxidase P33 Are Critical for Enzymatic Activity and Function.
Kuang et al. (2017)