› 3ut5

X-ray diffraction
2.73Å resolution

Tubulin-Colchicine-Ustiloxin: Stathmin-like domain complex

Released:
DOI: 10.2210/pdb3ut5/pdb
PDB entry 3ut5 coloured by chain, front view.
PDB entry 3ut5 coloured by chain, side view.
PDB entry 3ut5 coloured by chain, top view.
Source organisms:
Primary publication:
Structural plasticity of tubulin assembly probed by vinca-domain ligands.
Ranaivoson FM, Gigant B, Berritt S, Joullié M, Knossow M
Acta Crystallogr D Biol Crystallogr 68 927-34 (2012)
PMID: 22868758

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-112170 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50.04 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 142 amino acids
Theoretical weight: 16.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
Vinca tetrapeptide Chain: F
Molecule details ›
Chain: F
Length: 4 amino acids
Theoretical weight: 513 Da

Ligands and Environments

5 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand SO4 5 x SO4
Ligand GDP 2 x GDP
Ligand LOC 2 x LOC
2 modified residues:
Ligand 0EA 1 x 0EA
Ligand 0E5 1 x 0E5

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 64.81Å b: 128.87Å c: 254.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Antitubulin sulfonamides: The successful combination of an established drug class and a multifaceted target.
Vicente-Blázquez et al. (2019)
1 more

12 mentions without citation

Indole-Based Tubulin Inhibitors: Binding Modes and SARs Investigations.
Tang et al. (2022)
11 more