› 3uty

X-ray diffraction
2.37Å resolution

Crystal structure of bacteriorhodopsin mutant P50A/T46A

Released:
DOI: 10.2210/pdb3uty/pdb
PDB entry 3uty coloured by chain, front view.
PDB entry 3uty coloured by chain, side view.
PDB entry 3uty coloured by chain, top view.
Source organism: Halobacterium salinarum NRC-1
Primary publication:
Shifting hydrogen bonds may produce flexible transmembrane helices.
Cao Z, Bowie JU
Proc Natl Acad Sci U S A 109 8121-6 (2012)
PMID: 22566663

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 26.87 KDa
Source organism: Halobacterium salinarum NRC-1
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

2 bound ligands:
Ligand RET 2 x RET
Ligand D12 1 x D12
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 45.781Å b: 116.463Å c: 57.083Å
α: 90° β: 112.55° γ: 90°
R-values:
R R work R free
0.215 0.214 0.239
Expression system: Halobacterium salinarum

Quick links

Citations

7 review citations

Type IV pilin proteins: versatile molecular modules.
Giltner et al. (2012)
6 more

1 mention without citation

Shifting hydrogen bonds may produce flexible transmembrane helices.
Cao et al. (2012)