› 3uva

X-ray diffraction
2.69Å resolution

Crystal structure of L-rhamnose isomerase mutant W38F from Bacillus halodurans in complex with Mn

Released:
DOI: 10.2210/pdb3uva/pdb
PDB entry 3uva coloured by chain, front view.
PDB entry 3uva coloured by chain, side view.
PDB entry 3uva coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Primary publication:
Structure-based studies on the metal binding of two-metal-dependent sugar isomerases.
Prabhu P, Doan TN, Tiwari M, Singh R, Kim SC, Hong MK, Kang YC, Kang LW, Lee JK
FEBS J 281 3446-59 (2014)
PMID: 24925069

Function and Biology Details

Reaction catalysed: 
L-rhamnopyranose = L-rhamnulose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-rhamnose isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 424 amino acids
Theoretical weight: 49.04 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KCL9 (Residues: 1-418; Coverage: 100%)
Gene names: BH1552, rhaA
Sequence domains: L-rhamnose isomerase (RhaA)
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

1 bound ligand:
Ligand MN 8 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P21
Unit cell:
a: 83.374Å b: 164.779Å c: 92.816Å
α: 90° β: 117.01° γ: 90°
R-values:
R R work R free
0.19 0.185 0.266
Expression system: Escherichia coli

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Citations

1 review citation

Isomerases for biotransformation of D-hexoses.
Mu et al. (2015)
6 other articles