› 3uvf

X-ray diffraction
3Å resolution

Expanding LAGALIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space

Released:
DOI: 10.2210/pdb3uvf/pdb
PDB entry 3uvf coloured by chain, front view.
PDB entry 3uvf coloured by chain, side view.
PDB entry 3uvf coloured by chain, top view.
Source organism: Trichoderma reesei
Primary publication:
Expanding LAGLIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space.
Jacoby K, Metzger M, Shen BW, Certo MT, Jarjour J, Stoddard BL, Scharenberg AM
Nucleic Acids Res 40 4954-64 (2012)
PMID: 22334611

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-117009 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Intron-encoded DNA endonuclease I-HjeMI Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 29.62 KDa
Source organism: Trichoderma reesei
Expression system: Escherichia coli BL21(DE3)
Structure domains: Homing endonucleases
synthetic oligo Chains: C, E
Molecule details ›
Chains: C, E
Length: 28 nucleotides
Theoretical weight: 8.69 KDa
Source organism: Trichoderma reesei
Expression system: Not provided
synthetic oligo Chains: D, F
Molecule details ›
Chains: D, F
Length: 28 nucleotides
Theoretical weight: 8.52 KDa
Source organism: Trichoderma reesei
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand CA 6 x CA
Ligand PEG 4 x PEG
Ligand SO4 1 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21212
Unit cell:
a: 181.591Å b: 73.592Å c: 82.031Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.285 0.28 0.378
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Homing endonucleases: DNA scissors on a mission.
Hafez et al. (2012)
3 more

1 mention without citation

Redesign of extensive protein-DNA interfaces of meganucleases using iterative cycles of in vitro compartmentalization.
Takeuchi et al. (2014)