3uvv

X-ray diffraction
2.95Å resolution

Crystal Structure of the ligand binding domains of the thyroid receptor:retinoid X receptor complexed with 3,3',5 triiodo-L-thyronine and 9-cis retinoic acid

Released:
Source organisms:
Primary publication:
Structural basis for negative cooperativity within agonist-bound TR:RXR heterodimers.
Proc Natl Acad Sci U S A 109 6084-7 (2012)
PMID: 22474364

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-138059 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thyroid hormone receptor alpha Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 30.09 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04625 (Residues: 148-408; Coverage: 64%)
Gene names: NR1A1, THRA
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Retinoic acid receptor RXR-alpha Chain: B
Molecule details ›
Chain: B
Length: 244 amino acids
Theoretical weight: 27.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19793 (Residues: 225-462; Coverage: 52%)
Gene names: NR2B1, RXRA
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 129.13Å b: 165.32Å c: 85.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.25
Expression system: Escherichia coli BL21(DE3)