› 3uwq

X-ray diffraction
1.8Å resolution

1.80 Angstrom resolution crystal structure of orotidine 5'-phosphate decarboxylase from Vibrio cholerae O1 biovar eltor str. N16961 in complex with uridine-5'-monophosphate (UMP)

Released:
DOI: 10.2210/pdb3uwq/pdb
PDB entry 3uwq coloured by chain, front view.
PDB entry 3uwq coloured by chain, side view.
PDB entry 3uwq coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Halavaty AS, Minasov G, Winsor J, Shuvalova L, Kuhn M, Filippova EV, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate = UMP + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotidine 5'-phosphate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 27.78 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KQT7 (Residues: 1-231; Coverage: 100%)
Gene names: VC_1911, pyrF
Sequence domains: Orotidine 5'-phosphate decarboxylase / HUMPS family
Structure domains: Aldolase class I

Ligands and Environments

5 bound ligands:
Ligand U5P 2 x U5P
Ligand XPE 1 x XPE
Ligand PEG 4 x PEG
Ligand PGE 2 x PGE
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 52.14Å b: 96.944Å c: 104.082Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.178
Expression system: Escherichia coli BL21

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