› 3uzu

X-ray diffraction
1.75Å resolution

The structure of the Ribosomal RNA small subunit methyltransferase A from Burkholderia pseudomallei

Released:
DOI: 10.2210/pdb3uzu/pdb
PDB entry 3uzu coloured by chain, front view.
PDB entry 3uzu coloured by chain, side view.
PDB entry 3uzu coloured by chain, top view.
Source organism: Burkholderia pseudomallei
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase A Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 30.58 KDa
Source organism: Burkholderia pseudomallei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q63X76 (Residues: 1-275; Coverage: 100%)
Gene names: BPSL0661, ksgA, rsmA
Sequence domains: Ribosomal RNA adenine dimethylase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 91.19Å b: 49.34Å c: 61.16Å
α: 90° β: 95.91° γ: 90°
R-values:
R R work R free
0.182 0.18 0.217
Expression system: Escherichia coli

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