› 3v0f

X-ray diffraction
1.3Å resolution

Crystal structure of Ciona intestinalis voltage sensor-containing phosphatase (Ci-VSP), residues 241-576(C363S), form II

Released:
DOI: 10.2210/pdb3v0f/pdb
PDB entry 3v0f coloured by chain, front view.
PDB entry 3v0f coloured by chain, side view.
PDB entry 3v0f coloured by chain, top view.
Source organism: Ciona intestinalis
Primary publication:
A glutamate switch controls voltage-sensitive phosphatase function.
Liu L, Kohout SC, Xu Q, Müller S, Kimberlin CR, Isacoff EY, Minor DL
Nat Struct Mol Biol 19 633-41 (2012)
PMID: 22562138

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Voltage-sensor containing phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 38.83 KDa
Source organism: Ciona intestinalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4W8A1 (Residues: 241-576; Coverage: 58%)
Gene name: Ci-VSP
Sequence domains: C2 domain of PTEN tumour-suppressor protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 50.746Å b: 80.375Å c: 83.637Å
α: 90° β: 93.12° γ: 90°
R-values:
R R work R free
0.142 0.14 0.175
Expression system: Escherichia coli

Quick links

Citations

10 review citations

From one-photon to two-photon probes: "caged" compounds, actuators, and photoswitches.
Bort et al. (2013)
9 more

1 mention without citation

A glutamate switch controls voltage-sensitive phosphatase function.
Liu et al. (2012)