› 3v1a

X-ray diffraction
0.98Å resolution

Crystal structure of de novo designed MID1-apo1

Released:
DOI: 10.2210/pdb3v1a/pdb
PDB entry 3v1a coloured by chain, front view.
PDB entry 3v1a coloured by chain, side view.
PDB entry 3v1a coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Metal-mediated affinity and orientation specificity in a computationally designed protein homodimer.
Der BS, Machius M, Miley MJ, Mills JL, Szyperski T, Kuhlman B
J Am Chem Soc 134 375-85 (2012)
PMID: 22092237

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Computational design, MID1-apo1 Chain: A
Molecule details ›
Chain: A
Length: 48 amino acids
Theoretical weight: 5.5 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21
Structure domains: Rabenosyn, Rab binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 25.264Å b: 33.029Å c: 42.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.095 0.094 0.113
Expression system: Escherichia coli BL21

Quick links

Citations

23 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
22 more

5 mentions without citation

Strategies to control the binding mode of de novo designed protein interactions.
Der et al. (2013)
4 more