› 3v1b

X-ray diffraction
1.28Å resolution

Crystal structure of de novo designed MID1-apo2

Released:
DOI: 10.2210/pdb3v1b/pdb
PDB entry 3v1b coloured by chain, front view.
PDB entry 3v1b coloured by chain, side view.
PDB entry 3v1b coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Metal-mediated affinity and orientation specificity in a computationally designed protein homodimer.
Der BS, Machius M, Miley MJ, Mills JL, Szyperski T, Kuhlman B
J Am Chem Soc 134 375-85 (2012)
PMID: 22092237

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Computational design, MID1-apo2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 48 amino acids
Theoretical weight: 5.5 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21
Structure domains: Rabenosyn, Rab binding domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 25.405Å b: 41.931Å c: 67.159Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.203
Expression system: Escherichia coli BL21

Quick links

Citations

23 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
22 more

1 mention without citation

Strategies to control the binding mode of de novo designed protein interactions.
Der et al. (2013)