3v1h

X-ray diffraction
1.9Å resolution

Structure of the H258Y mutant of Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus

Released:

Function and Biology Details

Reaction catalysed:
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-155335 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.79 KDa
Source organism: Staphylococcus aureus subsp. aureus str. Newman
UniProt:
  • Canonical: P45723 (Residues: 13-312; Coverage: 100%)
Gene names: NWMN_0041, plc
Sequence domains: Phosphatidylinositol-specific phospholipase C, X domain
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 87.95Å b: 57.215Å c: 61.144Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.237