› 3v2g

X-ray diffraction
2.3Å resolution

Crystal structure of a dehydrogenase/reductase from Sinorhizobium meliloti 1021

Released:
DOI: 10.2210/pdb3v2g/pdb
PDB entry 3v2g coloured by chain, front view.
PDB entry 3v2g coloured by chain, side view.
PDB entry 3v2g coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Agarwal R, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, LaFleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 28.31 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92WA6 (Residues: 1-248; Coverage: 100%)
Gene name: SM_b20456
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P6222
Unit cell:
a: 138.088Å b: 138.088Å c: 77.207Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.167 0.205
Expression system: Escherichia coli BL21(DE3)

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