3v2u

X-ray diffraction
2.1Å resolution

Crystal structure of the yeast GAL regulon complex of the repressor, Gal80p, and the transducer, Gal3p, with galactose and ATP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-137617 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Galactose/lactose metabolism regulatory protein GAL80 Chains: A, B
Molecule details ›
Chains: A, B
Length: 438 amino acids
Theoretical weight: 48.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P04387 (Residues: 1-435; Coverage: 100%)
Gene names: GAL80, YM9827.01, YM9958.12, YML051W
Sequence domains: Oxidoreductase family, NAD-binding Rossmann fold
Structure domains:
Protein GAL3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 520 amino acids
Theoretical weight: 58.15 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P13045 (Residues: 2-520; Coverage: 100%)
Gene names: GAL3, YD8119.14, YDR009W
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P1
Unit cell:
a: 82.186Å b: 83.221Å c: 92.629Å
α: 114.04° β: 92.88° γ: 90.37°
R-values:
R R work R free
0.15 0.15 0.196
Expression system: Escherichia coli