› 3v3o

X-ray diffraction
2.9Å resolution

Crystal structure of TetX2 T280A: an adaptive mutant in complex with tigecycline

Released:
DOI: 10.2210/pdb3v3o/pdb
PDB entry 3v3o coloured by chain, front view.
PDB entry 3v3o coloured by chain, side view.
PDB entry 3v3o coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron
Entry authors: Walkiewicz K, Shamoo Y

Function and Biology Details

Reaction catalysed: 
Tetracycline + NADPH + O(2) = 11a-hydroxytetracycline + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin-dependent monooxygenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 378 amino acids
Theoretical weight: 42.5 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93L51 (Residues: 11-388; Coverage: 97%)
Gene name: tetX2
Sequence domains: FAD binding domain
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
2 bound ligands:
Ligand SO4 6 x SO4
Ligand T1C 4 x T1C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P1
Unit cell:
a: 68.126Å b: 80.08Å c: 87.238Å
α: 111.02° β: 89.97° γ: 92.97°
R-values:
R R work R free
0.235 0.232 0.298
Expression system: Escherichia coli

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