› 3v3s

X-ray diffraction
1.9Å resolution

Crystal structure of GES-18

Released:
DOI: 10.2210/pdb3v3s/pdb
Model geometry
Fit model/data
PDB entry 3v3s coloured by chain, front view.
PDB entry 3v3s coloured by chain, side view.
PDB entry 3v3s coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
GES-18, a new carbapenem-hydrolyzing GES-Type β-lactamase from Pseudomonas aeruginosa that contains Ile80 and Ser170 residues.
Bebrone C, Bogaerts P, Delbrück H, Bennink S, Kupper MB, Rezende de Castro R, Glupczynski Y, Hoffmann KM
Antimicrob Agents Chemother 57 396-401 (2013)
PMID: 23114760

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 31.24 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: H2ERK4 (Residues: 1-287; Coverage: 100%)
Gene name: blaGES-18
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P212121
Unit cell:
a: 76.184Å b: 80.506Å c: 87.748Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.196
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwide.
Nordmann et al. (2014)
6 more

3 mentions without citation

Structural and Functional Aspects of Class A Carbapenemases.
Naas et al. (2016)
2 more