› 3v47

X-ray diffraction
2.47Å resolution

Crystal structure of the N-terminal fragment of zebrafish TLR5 in complex with Salmonella flagellin

Released:
DOI: 10.2210/pdb3v47/pdb
PDB entry 3v47 coloured by chain, front view.
PDB entry 3v47 coloured by chain, side view.
PDB entry 3v47 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of TLR5-flagellin recognition and signaling.
Yoon SI, Kurnasov O, Natarajan V, Hong M, Gudkov AV, Osterman AL, Wilson IA
Science 335 859-64 (2012)
PMID: 22344444

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109810 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
LRRNT domain-containing protein; TIR domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 51.17 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: B3DIN1 (Residues: 22-390; Coverage: 43%)
  • Canonical: Q4G1L2 (Residues: 126-200; Coverage: 30%)
Gene names: VLRB, tlr5b
Sequence domains:
Flagellin Chains: C, D
Molecule details ›
Chains: C, D
Length: 425 amino acids
Theoretical weight: 44.55 KDa
Source organism: Salmonella enterica subsp. enterica serovar Dublin
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06971 (Residues: 48-466; Coverage: 83%)
Gene names: fliC, fliC1
Sequence domains:
Structure domains: f41 fragment of flagellin, N-terminal domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 58.406Å b: 181.495Å c: 186.367Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.259
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

95 review citations

The history of Toll-like receptors - redefining innate immunity.
O'Neill et al. (2013)
94 more

27 mentions without citation

The critical role of toll-like receptors--From microbial recognition to autoimmunity: A comprehensive review.
Jiménez-Dalmaroni et al. (2016)
26 more