› 3v4d

X-ray diffraction
1.95Å resolution

Crystal structure of RutC protein a member of the YjgF family from E.coli

Released:
DOI: 10.2210/pdb3v4d/pdb
PDB entry 3v4d coloured by chain, front view.
PDB entry 3v4d coloured by chain, side view.
PDB entry 3v4d coloured by chain, top view.
Source organism: Escherichia coli O6
Primary publication:
Structure of Escherichia coli RutC, a member of the YjgF family and putative aminoacrylate peracid reductase of the rut operon.
Knapik AA, Petkowski JJ, Otwinowski Z, Cymborowski MT, Cooper DR, Chruszcz M, Krajewska WM, Minor W
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 1294-9 (2012)
PMID: 23143235

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142746 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-aminoacrylate deaminase RutC Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 134 amino acids
Theoretical weight: 14.62 KDa
Source organism: Escherichia coli O6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFQ6 (Residues: 1-128; Coverage: 100%)
Gene names: c1147, rutC
Sequence domains: Endoribonuclease L-PSP
Structure domains: RutC-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21212
Unit cell:
a: 92.255Å b: 179.181Å c: 45.217Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.217
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Modulators of a robust and efficient metabolism: Perspective and insights from the Rid superfamily of proteins.
Fulton et al. (2023)
6 other articles

5 mentions without citation

RidA Proteins Protect against Metabolic Damage by Reactive Intermediates.
Irons et al. (2020)
4 more