› 3v6t

X-ray diffraction
1.85Å resolution

Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom

Released:
DOI: 10.2210/pdb3v6t/pdb
PDB entry 3v6t coloured by chain, front view.
PDB entry 3v6t coloured by chain, side view.
PDB entry 3v6t coloured by chain, top view.
Source organism: Xanthomonas
Primary publication:
Structural basis for sequence-specific recognition of DNA by TAL effectors.
Deng D, Yan C, Pan X, Mahfouz M, Wang J, Zhu JK, Shi Y, Yan N
Science 335 720-3 (2012)
PMID: 22223738

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-117026 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
dHax3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 51.74 KDa
Source organism: Xanthomonas
Expression system: Escherichia coli
DNA (5'-D(*TP*GP*TP*CP*CP*CP*TP*TP*TP*AP*TP*CP*TP*CP*TP*CP*T)-3') Chains: G, I
Molecule details ›
Chains: G, I
Length: 17 nucleotides
Theoretical weight: 5.07 KDa
Source organism: Xanthomonas
Expression system: Not provided
DNA (5'-D(*AP*GP*AP*GP*AP*GP*AP*TP*AP*AP*AP*GP*GP*GP*AP*CP*A)-3') Chains: H, J
Molecule details ›
Chains: H, J
Length: 17 nucleotides
Theoretical weight: 5.34 KDa
Source organism: Xanthomonas
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 81.719Å b: 87.679Å c: 88.494Å
α: 90° β: 103.04° γ: 90°
R-values:
R R work R free
0.206 0.205 0.222
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

130 review citations

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering.
Gaj et al. (2013)
129 more

18 mentions without citation

TAL effectors: function, structure, engineering and applications.
Mak et al. (2013)
17 more