› 3v71

X-ray diffraction
2.9Å resolution

Crystal structure of PUF-6 in complex with 5BE13 RNA

Released:
DOI: 10.2210/pdb3v71/pdb
PDB entry 3v71 coloured by chain, front view.
PDB entry 3v71 coloured by chain, side view.
PDB entry 3v71 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Divergence of PUF protein specificity through variations in an RNA-binding pocket
Qiu C, Kershner A, Wang Y, Holley CH, Wilinski D, Keles S, Kimble J, Wickens M, Hall TMT
J. Biol. Chem. (2012)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129185 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Pumilio domain-containing protein 7 Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 43.7 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O44169 (Residues: 76-453; Coverage: 78%)
Gene names: B0273.2, puf-7
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
RNA (5'-R(*CP*UP*CP*UP*GP*UP*AP*UP*CP*UP*UP*GP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.03 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 67.806Å b: 87.836Å c: 129.782Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.241
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links