› 3v75

X-ray diffraction
1.4Å resolution

Crystal structure of putative orotidine 5'-phosphate decarboxylase from Streptomyces avermitilis ma-4680

Released:
DOI: 10.2210/pdb3v75/pdb
PDB entry 3v75 coloured by chain, front view.
PDB entry 3v75 coloured by chain, side view.
PDB entry 3v75 coloured by chain, top view.
Source organism: Streptomyces avermitilis
Entry authors: Stogios PJ, Xu X, Cui H, Kudritska M, Tan K, Edwards A, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate = UMP + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182651 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotidine 5'-phosphate decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 31.68 KDa
Source organism: Streptomyces avermitilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q827Q5 (Residues: 1-280; Coverage: 100%)
Gene names: SAVERM_6869, pyrF
Sequence domains: Orotidine 5'-phosphate decarboxylase / HUMPS family
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 77.882Å b: 83.342Å c: 44.081Å
α: 90° β: 110.52° γ: 90°
R-values:
R R work R free
0.149 0.148 0.172
Expression system: Escherichia coli BL21

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