3v7e

X-ray diffraction
2.8Å resolution

Crystal structure of YbxF bound to the SAM-I riboswitch aptamer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-155459 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-binding protein YbxF Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 8.4 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P46350 (Residues: 2-82; Coverage: 99%)
Gene names: BSU01090, rplGB, rulS, ybaB, ybxF
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
SAM-I riboswitch aptamer with an engineered helix P3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 126 nucleotides
Theoretical weight: 40.8 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Not provided
Sequence domains: SAM riboswitch (S box leader)

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 191.762Å b: 54.305Å c: 106.366Å
α: 90° β: 116.56° γ: 90°
R-values:
R R work R free
0.218 0.218 0.274
Expression systems:
  • Escherichia coli
  • Not provided