› 3v8b

X-ray diffraction
2.7Å resolution

Crystal Structure of a 3-ketoacyl-ACP reductase from Sinorhizobium meliloti 1021

Released:
DOI: 10.2210/pdb3v8b/pdb
PDB entry 3v8b coloured by chain, front view.
PDB entry 3v8b coloured by chain, side view.
PDB entry 3v8b coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Kumaran D, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, Lafleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187934 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dehydrogenase, possibly 3-oxoacyl-[acyl-carrier protein] reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 283 amino acids
Theoretical weight: 30.35 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92U42 (Residues: 1-260; Coverage: 100%)
Gene name: SM_b21474
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 114.787Å b: 143.916Å c: 66.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.187 0.255
Expression system: Escherichia coli BL21(DE3)

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