› 3vay

X-ray diffraction
1.98Å resolution

Crystal structure of 2-Haloacid Dehalogenase from Pseudomonas syringae pv. Tomato DC3000

Released:
DOI: 10.2210/pdb3vay/pdb
PDB entry 3vay coloured by chain, front view.
PDB entry 3vay coloured by chain, side view.
PDB entry 3vay coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Primary publication:
Structure of 2-haloacid dehalogenase from Pseudomonas syringae pv. tomato DC3000.
Hou Z, Zhang H, Li M, Chang W
Acta Crystallogr D Biol Crystallogr 69 1108-14 (2013)
PMID: 23695255

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HAD-superfamily hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 25.57 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88B12 (Residues: 1-230; Coverage: 100%)
Gene name: PSPTO_0221
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand IOD 15 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 44.325Å b: 92.274Å c: 70.191Å
α: 90° β: 108.13° γ: 90°
R-values:
R R work R free
0.207 0.193 0.224
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Diversification in the inositol tris/tetrakisphosphate kinase (ITPK) family: crystal structure and enzymology of the outlier AtITPK4.
Whitfield et al. (2023)

1 mention without citation

In Silico Analysis on the Interaction of Haloacid Dehalogenase from Bacillus cereus IndB1 with 2-Chloroalkanoic Acid Substrates.
Ratnaningsih et al. (2022)