› 3vaz

X-ray diffraction
3.19Å resolution

Crystal structure of Staphylococcal GAPDH1 in a hexagonal space group

Released:
DOI: 10.2210/pdb3vaz/pdb
PDB entry 3vaz coloured by chain, front view.
PDB entry 3vaz coloured by chain, side view.
PDB entry 3vaz coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus MRSA252
Entry authors: Roychowdhury A, Mukherjee S, Dutta D, Das AK

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase 1 Chains: A, B, O, P, Q, R
Molecule details ›
Chains: A, B, O, P, Q, R
Length: 344 amino acids
Theoretical weight: 37.29 KDa
Source organism: Staphylococcus aureus subsp. aureus MRSA252
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6GIL8 (Residues: 1-336; Coverage: 100%)
Gene names: SAR0828, gap, gapA, gapA1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 6 x NAD
2 bound ligands:
Ligand PO4 6 x PO4
Ligand DGY 2 x DGY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6222
Unit cell:
a: 155.389Å b: 155.389Å c: 317.849Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.202 0.277
Expression system: Escherichia coli

Quick links