› 3vba

X-ray diffraction
2Å resolution

Crystal structure of methanogen 3-isopropylmalate isomerase small subunit

Released:
DOI: 10.2210/pdb3vba/pdb
PDB entry 3vba coloured by chain, front view.
PDB entry 3vba coloured by chain, side view.
PDB entry 3vba coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Crystal structure of LeuD from Methanococcus jannaschii.
Lee EH, Cho YW, Hwang KY
Biochem Biophys Res Commun 419 160-4 (2012)
PMID: 22326391

Function and Biology Details

Reactions catalysed: 
(R)-malate = maleate + H(2)O
(1a) (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H(2)O
(R)-2-methylmalate = 2-methylmaleate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopropylmalate/citramalate isomerase small subunit Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 176 amino acids
Theoretical weight: 19.47 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58673 (Residues: 1-168; Coverage: 100%)
Gene names: MJ1277, leuD
Sequence domains: Aconitase C-terminal domain
Structure domains: Aconitase, domain 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 33.836Å b: 107.138Å c: 288.714Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.244
Expression system: Escherichia coli

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Citations

1 review citation

Stereochemistry of enzymatic water addition to C=C bonds.
Chen et al. (2015)
3 other articles