› 3vcd

X-ray diffraction
2.35Å resolution

Computationally Designed Self-assembling Octahedral Cage protein, O333, Crystallized in space group R32

Released:
DOI: 10.2210/pdb3vcd/pdb
Model geometry
Fit model/data
PDB entry 3vcd coloured by chain, front view.
PDB entry 3vcd coloured by chain, side view.
PDB entry 3vcd coloured by chain, top view.
Source organism: Salmonella enterica
Primary publication:
Computational design of self-assembling protein nanomaterials with atomic level accuracy.
King NP, Sheffler W, Sawaya MR, Vollmar BS, Sumida JP, André I, Gonen T, Yeates TO, Baker D
Science 336 1171-4 (2012)
PMID: 22654060

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial microcompartment shell protein PduT Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 192 amino acids
Theoretical weight: 19.96 KDa
Source organism: Salmonella enterica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9XDM8 (Residues: 1-184; Coverage: 100%)
Gene names: STM2054, pduT
Sequence domains: BMC domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand SO4 26 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 137.92Å b: 137.92Å c: 560.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.213
Expression system: Escherichia coli

Quick links

Citations

100 review citations

Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes.
Wei et al. (2013)
99 more

6 mentions without citation

Protein-based antigen presentation platforms for nanoparticle vaccines.
Nguyen et al. (2021)
5 more