› 3vco

X-ray diffraction
1.95Å resolution

Schistosoma mansoni Dihydrofolate reductase

Released:
DOI: 10.2210/pdb3vco/pdb
PDB entry 3vco coloured by chain, front view.
PDB entry 3vco coloured by chain, side view.
PDB entry 3vco coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
Structure and kinetics assays of recombinant Schistosoma mansoni dihydrofolate reductase.
Serrão VHB, Romanello L, Cassago A, de Souza JRT, Cheleski J, DeMarco R, Brandão-Neto J, Pereira HD
Acta Trop 170 190-196 (2017)
PMID: 28288799

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124460 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 22.68 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G4VJD6 (Residues: 1-181; Coverage: 97%)
Gene names: DHFR, Smp_175230
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 107.352Å b: 107.352Å c: 33.458Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.38 0.201 0.25
Expression system: Escherichia coli BL21(DE3)

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Citations

2 mentions without citation

Molecular Characteristics and Serodiagnostic Potential of Dihydrofolate Reductase from Echinococcus granulosus.
Song et al. (2017)
1 more