› 3vfd

X-ray diffraction
3.3Å resolution

Human spastin AAA domain

Released:
DOI: 10.2210/pdb3vfd/pdb
PDB entry 3vfd coloured by chain, front view.
PDB entry 3vfd coloured by chain, side view.
PDB entry 3vfd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human spastin AAA domain.
Taylor JL, White SR, Lauring B, Kull FJ
J Struct Biol 179 133-7 (2012)
PMID: 22446388

Function and Biology Details

Reaction catalysed: 
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spastin Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 42.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9UBP0 (Residues: 228-616; Coverage: 63%)
Gene names: ADPSP, FSP2, KIAA1083, SPAST, SPG4
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P65
Unit cell:
a: 88.5Å b: 88.5Å c: 88.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.206 0.275
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Microtubule-severing enzymes: From cellular functions to molecular mechanism.
McNally et al. (2018)
12 other articles

6 mentions without citation

Meiotic Clade AAA ATPases: Protein Polymer Disassembly Machines.
Monroe et al. (2016)
5 more