› 3vfz

X-ray diffraction
1.9Å resolution

Crystal structure of -35 promoter binding domain of SigD of Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb3vfz/pdb
PDB entry 3vfz coloured by chain, front view.
PDB entry 3vfz coloured by chain, side view.
PDB entry 3vfz coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Mycobacterium tuberculosis RsdA provides a conformational rationale for selective regulation of σ-factor activity by proteolysis.
Jaiswal RK, Prabha TS, Manjeera G, Gopal B
Nucleic Acids Res 41 3414-23 (2013)
PMID: 23314154

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-161472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ECF RNA polymerase sigma factor SigD Chains: A, B
Molecule details ›
Chains: A, B
Length: 86 amino acids
Theoretical weight: 9.47 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGG9 (Residues: 141-212; Coverage: 34%)
Gene names: MTCY78.15, Rv3414c, sigD
Sequence domains: Sigma-70, region 4
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 80.43Å b: 35.7Å c: 50.92Å
α: 90° β: 122.59° γ: 90°
R-values:
R R work R free
0.2 0.198 0.238
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
2 more

1 mention without citation

Mycobacterium tuberculosis RsdA provides a conformational rationale for selective regulation of σ-factor activity by proteolysis.
Jaiswal et al. (2013)