› 3vh9

X-ray diffraction
1.29Å resolution

Crystal structure of Aeromonas proteolytica aminopeptidase complexed with 8-quinolinol

Released:
DOI: 10.2210/pdb3vh9/pdb
PDB entry 3vh9 coloured by chain, front view.
PDB entry 3vh9 coloured by chain, side view.
PDB entry 3vh9 coloured by chain, top view.
Source organism: Vibrio proteolyticus
Primary publication:
Potent inhibition of dinuclear zinc(II) peptidase, an aminopeptidase from Aeromonas proteolytica, by 8-quinolinol derivatives: inhibitor design based on Zn2+ fluorophores, kinetic, and X-ray crystallographic study.
Hanaya K, Suetsugu M, Saijo S, Yamato I, Aoki S
J Biol Inorg Chem 17 517-29 (2012)
PMID: 22311113

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial leucyl aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 32.24 KDa
Source organism: Vibrio proteolyticus
UniProt:
  • Canonical: Q01693 (Residues: 107-405; Coverage: 62%)
Sequence domains: Peptidase family M28
Structure domains: Zn peptidases

Ligands and Environments

6 bound ligands:
Ligand ZN 2 x ZN
Ligand HQY 1 x HQY
Ligand NA 9 x NA
Ligand CL 9 x CL
Ligand SCN 2 x SCN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P6122
Unit cell:
a: 108.463Å b: 108.463Å c: 90.737Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.132 0.131 0.153

Quick links

Citations

6 citation in other articles

Dipicolinic Acid Derivatives as Inhibitors of New Delhi Metallo-β-lactamase-1.
Chen et al. (2017)
5 more

3 mentions without citation

Potent inhibition of dinuclear zinc(II) peptidase, an aminopeptidase from Aeromonas proteolytica, by 8-quinolinol derivatives: inhibitor design based on Zn2+ fluorophores, kinetic, and X-ray crystallographic study.
Hanaya et al. (2012)
2 more