› 3vj9

X-ray diffraction
1.52Å resolution

Crystal structure of the human squalene synthase

Released:
DOI: 10.2210/pdb3vj9/pdb
PDB entry 3vj9 coloured by chain, front view.
PDB entry 3vj9 coloured by chain, side view.
PDB entry 3vj9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Binding modes of zaragozic acid A to human squalene synthase and staphylococcal dehydrosqualene synthase.
Liu CI, Jeng WY, Chang WJ, Ko TP, Wang AH
J Biol Chem 287 18750-7 (2012)
PMID: 22474324

Function and Biology Details

Reaction catalysed: 
(1a) 2 farnesyl diphosphate = diphosphate + presqualene diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Squalene synthase Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37268 (Residues: 31-370; Coverage: 82%)
Gene name: FDFT1
Sequence domains: Squalene/phytoene synthase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 51.776Å b: 76.657Å c: 82.501Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.16 0.214
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Human isoprenoid synthase enzymes as therapeutic targets.
Park et al. (2014)
1 more

5 mentions without citation

Binding modes of zaragozic acid A to human squalene synthase and staphylococcal dehydrosqualene synthase.
Liu et al. (2012)
4 more