› 3vjf

X-ray diffraction
2.2Å resolution

Crystal structure of de novo 4-helix bundle protein WA20

Released:
DOI: 10.2210/pdb3vjf/pdb
PDB entry 3vjf coloured by chain, front view.
PDB entry 3vjf coloured by chain, side view.
PDB entry 3vjf coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Domain-swapped dimeric structure of a stable and functional de novo four-helix bundle protein, WA20.
Arai R, Kobayashi N, Kimura A, Sato T, Matsuo K, Wang AF, Platt JM, Bradley LH, Hecht MH
J Phys Chem B 116 6789-97 (2012)
PMID: 22397676

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164181 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WA20 Chains: A, B
Molecule details ›
Chains: A, B
Length: 102 amino acids
Theoretical weight: 12.94 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Designed four-helix bundle protein

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 65.95Å b: 102.858Å c: 31.344Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.233 0.255
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Design and construction of self-assembling supramolecular protein complexes using artificial and fusion proteins as nanoscale building blocks.
Kobayashi et al. (2017)
2 more

6 mentions without citation

Hierarchical design of artificial proteins and complexes toward synthetic structural biology.
Arai R. (2018)
5 more