› 3vlf

X-ray diffraction
3.8Å resolution

Crystal structure of yeast proteasome interacting protein

Released:
DOI: 10.2210/pdb3vlf/pdb
PDB entry 3vlf coloured by chain, front view.
PDB entry 3vlf coloured by chain, side view.
PDB entry 3vlf coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis for specific recognition of Rpt1p, an ATPase subunit of 26 S proteasome, by proteasome-dedicated chaperone Hsm3p.
Takagi K, Kim S, Yukii H, Ueno M, Morishita R, Endo Y, Kato K, Tanaka K, Saeki Y, Mizushima T
J Biol Chem 287 12172-82 (2012)
PMID: 22334676

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152575 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA mismatch repair protein HSM3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 500 amino acids
Theoretical weight: 58.24 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38348 (Residues: 1-480; Coverage: 100%)
Gene names: HSM3, YBR1740, YBR272C
Sequence domains:
Structure domains:
26S proteasome regulatory subunit 7 homolog Chains: B, D
Molecule details ›
Chains: B, D
Length: 88 amino acids
Theoretical weight: 10.3 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P33299 (Residues: 381-467; Coverage: 19%)
Gene names: CIM5, RPT1, YKL145W, YTA3
Sequence domains: AAA+ lid domain
Structure domains: Helicase, Ruva Protein; domain 3

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P6522
Unit cell:
a: 187.282Å b: 187.282Å c: 379.574Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.25 0.278
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The ubiquitin-proteasome system of Saccharomyces cerevisiae.
Finley et al. (2012)
8 more

3 mentions without citation

Molecular architecture and assembly of the eukaryotic proteasome.
Tomko et al. (2013)
2 more