› 3vmf

X-ray diffraction
2.3Å resolution

Archaeal protein

Released:
DOI: 10.2210/pdb3vmf/pdb
Model geometry
Fit model/data
PDB entry 3vmf coloured by chain, front view.
PDB entry 3vmf coloured by chain, side view.
PDB entry 3vmf coloured by chain, top view.
Source organism: Aeropyrum pernix K1
Primary publication:
Structural basis for translation termination by archaeal RF1 and GTP-bound EF1α complex.
Kobayashi K, Saito K, Ishitani R, Ito K, Nureki O
OpenAccess logo Nucleic Acids Res 40 9319-28 (2012)
PMID: 22772989

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195439 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Elongation factor 1-alpha Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 49.02 KDa
Source organism: Aeropyrum pernix K1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9YAV0 (Residues: 1-437; Coverage: 100%)
Gene names: APE_1844, tuf
Sequence domains:
Structure domains:
Peptide chain release factor subunit 1 Chain: B
Molecule details ›
Chain: B
Length: 373 amino acids
Theoretical weight: 42.01 KDa
Source organism: Aeropyrum pernix K1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9YAF1 (Residues: 1-373; Coverage: 100%)
Gene names: APE_1988.1, prf1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 176.567Å b: 55.169Å c: 90.689Å
α: 90° β: 101.61° γ: 90°
R-values:
R R work R free
0.204 0.201 0.261
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Origin and Evolution of Release Factors: Implications for Translation Termination, Ribosome Rescue, and Quality Control Pathways.
Burroughs et al. (2019)
1 more

11 mentions without citation

Trm112, a Protein Activator of Methyltransferases Modifying Actors of the Eukaryotic Translational Apparatus.
Bourgeois et al. (2017)
10 more