› 3vn3

X-ray diffraction
0.95Å resolution

Fungal antifreeze protein exerts hyperactivity by constructing an inequable beta-helix

Released:
DOI: 10.2210/pdb3vn3/pdb
PDB entry 3vn3 coloured by chain, front view.
PDB entry 3vn3 coloured by chain, side view.
PDB entry 3vn3 coloured by chain, top view.
Source organism: Typhula ishikariensis
Primary publication:
Ice-binding site of snow mold fungus antifreeze protein deviates from structural regularity and high conservation.
Kondo H, Hanada Y, Sugimoto H, Hoshino T, Garnham CP, Davies PL, Tsuda S
Proc Natl Acad Sci U S A 109 9360-5 (2012)
PMID: 22645341

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181146 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ice-binding protein K3-B1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 22.31 KDa
Source organism: Typhula ishikariensis
UniProt:
  • Canonical: Q76CE6 (Residues: 21-243; Coverage: 100%)
Gene name: K3-B1
Sequence domains: Ice-binding-like

Ligands and Environments

1 bound ligand:
Ligand EDO 7 x EDO
1 modified residue:
Ligand IYR 2 x IYR

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P1
Unit cell:
a: 38.89Å b: 41.63Å c: 70.25Å
α: 81.53° β: 83.13° γ: 62.43°
R-values:
R R work R free
0.113 0.112 0.129

Quick links

Citations

9 review citations

Ice-Binding Proteins and Their Function.
Bar Dolev et al. (2016)
8 more

9 mentions without citation

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant.
Kim et al. (2017)
8 more