› 3vnb

X-ray diffraction
1.5Å resolution

Structural insights into small RNA sorting and mRNA binding by Arabidopsis Ago domains

Released:
DOI: 10.2210/pdb3vnb/pdb
PDB entry 3vnb coloured by chain, front view.
PDB entry 3vnb coloured by chain, side view.
PDB entry 3vnb coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insights into small RNA sorting and mRNA target binding by Arabidopsis Argonaute Mid domains.
Zha X, Xia Q, Yuan YA
FEBS Lett 586 3200-7 (2012)
PMID: 22771475

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute 1 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.61 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O04379 (Residues: 592-683, 684-739; Coverage: 14%)
Gene names: AGO1, At1g48410, F11A17.3, T1N15.2
Sequence domains: Mid domain of argonaute
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 36.869Å b: 61.096Å c: 67.085Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.215
Expression system: Escherichia coli

Quick links

Citations

3 review citations

microRNA biogenesis and turnover in plants.
Rogers et al. (2012)
2 more