› 3vnp

X-ray diffraction
2.4Å resolution

Crystal structure of hypothetical protein (GK2848) from Geobacillus Kaustophilus

Released:
DOI: 10.2210/pdb3vnp/pdb
PDB entry 3vnp coloured by chain, front view.
PDB entry 3vnp coloured by chain, side view.
PDB entry 3vnp coloured by chain, top view.
Source organism: Geobacillus kaustophilus HTA426
Entry authors: Karthe PP, Kumarevel TS, Kuramitsu S, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177363 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 183 amino acids
Theoretical weight: 20.04 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5KW03 (Residues: 1-182; Coverage: 100%)
Gene name: GK2848
Sequence domains: Bacterial transferase hexapeptide (six repeats)
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

2 bound ligands:
Ligand ACY 3 x ACY
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21212
Unit cell:
a: 137.978Å b: 58.704Å c: 94.393Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.247
Expression system: Escherichia coli

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