› 3vor

X-ray diffraction
0.9Å resolution

Crystal Structure Analysis of the CofA

Released:
DOI: 10.2210/pdb3vor/pdb
PDB entry 3vor coloured by chain, front view.
PDB entry 3vor coloured by chain, side view.
PDB entry 3vor coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the CFA/III major pilin subunit CofA from human enterotoxigenic Escherichia coli determined at 0.90 Å resolution by sulfur-SAD phasing.
Fukakusa S, Kawahara K, Nakamura S, Iwashita T, Baba S, Nishimura M, Kobayashi Y, Honda T, Iida T, Taniguchi T, Ohkubo T
Acta Crystallogr D Biol Crystallogr 68 1418-29 (2012)
PMID: 22993096

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CFA/III pilin Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 18.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59393 (Residues: 59-238; Coverage: 76%)
Gene name: cofA
Sequence domains: Toxin-coregulated pilus subunit TcpA
Structure domains: TcpA-like pilin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 33.82Å b: 47.71Å c: 42.45Å
α: 90° β: 107.52° γ: 90°
R-values:
R R work R free
0.121 0.12 0.13
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium.
Neuhaus et al. (2020)
4 more

5 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
4 more