› 3vpc

X-ray diffraction
1.87Å resolution

ArgX from Sulfolobus tokodaii complexed with ADP

Released:
DOI: 10.2210/pdb3vpc/pdb
PDB entry 3vpc coloured by chain, front view.
PDB entry 3vpc coloured by chain, side view.
PDB entry 3vpc coloured by chain, top view.
Source organism: Sulfurisphaera tokodaii
Primary publication:
Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus.
Ouchi T, Tomita T, Horie A, Yoshida A, Takahashi K, Nishida H, Lassak K, Taka H, Mineki R, Fujimura T, Kosono S, Nishiyama C, Masui R, Kuramitsu S, Albers SV, Kuzuyama T, Nishiyama M
Nat Chem Biol 9 277-83 (2013)
PMID: 23434852

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--LysW ligase ArgX Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 282 amino acids
Theoretical weight: 31.57 KDa
Source organism: Sulfurisphaera tokodaii
Expression system: Sulfurisphaera tokodaii str. 7
UniProt:
  • Canonical: Q970U6 (Residues: 1-282; Coverage: 100%)
Gene names: STK_15050, argE, argX
Sequence domains: RimK-like ATP-grasp domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 4 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P1
Unit cell:
a: 63.266Å b: 67.845Å c: 80.032Å
α: 87.77° β: 75.29° γ: 67.03°
R-values:
R R work R free
0.201 0.198 0.253
Expression system: Sulfurisphaera tokodaii str. 7

Quick links

Citations

4 review citations

The N-Acetylglutamate Synthase Family: Structures, Function and Mechanisms.
Shi et al. (2015)
3 more

1 mention without citation

Structural polymorphism of the Escherichia coli poly-α-L-glutamate synthetase RimK.
Arimura et al. (2018)