› 3vpy

X-ray diffraction
1.7Å resolution

Crystal structure of Arabidopsis DDL FHA domain

Released:
DOI: 10.2210/pdb3vpy/pdb
PDB entry 3vpy coloured by chain, front view.
PDB entry 3vpy coloured by chain, side view.
PDB entry 3vpy coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structure of Arabidopsis thaliana Dawdle forkhead-associated domain reveals a conserved phospho-threonine recognition cleft for dicer-like 1 binding.
Machida S, Yuan YA
Mol Plant 6 1290-300 (2013)
PMID: 23313986

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FHA domain-containing protein DDL Chain: A
Molecule details ›
Chain: A
Length: 145 amino acids
Theoretical weight: 17.53 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8W4D8 (Residues: 180-310; Coverage: 42%)
Gene names: At3g20550, DDL, K10D20.9
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 48.564Å b: 48.709Å c: 54.688Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.188 0.231
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Biogenesis, turnover, and mode of action of plant microRNAs.
Rogers et al. (2013)
14 more

2 mentions without citation

Structural insights into the recognition of phosphopeptide by the FHA domain of kanadaptin.
Xu et al. (2014)
1 more