› 3vqg

X-ray diffraction
1.35Å resolution

Crystal Structure Analysis of the PDZ Domain Derived from the Tight Junction Regulating Protein

Released:
DOI: 10.2210/pdb3vqg/pdb
PDB entry 3vqg coloured by chain, front view.
PDB entry 3vqg coloured by chain, side view.
PDB entry 3vqg coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Akiyoshi Y, Hamada D, Goda N, Tenno T, Narita H, Nakagawa A, Furuse M, Suzuki M, Hiroaki H

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130688 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase LNX Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.39 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O70263 (Residues: 381-467; Coverage: 12%)
Gene names: Lnx, Lnx1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
Immunoglobulin superfamily member 5 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.09 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q7TSN7 (Residues: 363-370; Coverage: 2%)
Gene names: Igsf5, Jam4

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: C2
Unit cell:
a: 60.852Å b: 50.768Å c: 33.136Å
α: 90° β: 120.08° γ: 90°
R-values:
R R work R free
0.172 0.17 0.207
Expression systems:
  • Escherichia coli
  • Not provided

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