› 3vtr

X-ray diffraction
2.5Å resolution

Crystal Structure of insect beta-N-acetyl-D-hexosaminidase OfHex1 E328A complexed with TMG-chitotriomycin

Released:
DOI: 10.2210/pdb3vtr/pdb
PDB entry 3vtr coloured by chain, front view.
PDB entry 3vtr coloured by chain, side view.
PDB entry 3vtr coloured by chain, top view.
Source organism: Ostrinia furnacalis
Primary publication:
Structural insights into cellulolytic and chitinolytic enzymes revealing crucial residues of insect β-N-acetyl-D-hexosaminidase.
Liu T, Zhou Y, Chen L, Chen W, Liu L, Shen X, Zhang W, Zhang J, Yang Q
PLoS One 7 e52225 (2012)
PMID: 23300622

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-170422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitooligosaccharidolytic beta-N-acetylglucosaminidase Chain: A
Molecule details ›
Chain: A
Length: 572 amino acids
Theoretical weight: 65.49 KDa
Source organism: Ostrinia furnacalis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q06GJ0 (Residues: 23-594; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, TMX
Ligand NAG 3 x NAG
Ligand TMX 1 x TMX
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3221
Unit cell:
a: 107.937Å b: 107.937Å c: 174.948Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.217 0.244
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Chitinase from Thermomyces lanuginosus SSBP and its biotechnological applications.
Khan et al. (2015)
7 other articles

2 mentions without citation

Structural insights into cellulolytic and chitinolytic enzymes revealing crucial residues of insect β-N-acetyl-D-hexosaminidase.
Liu et al. (2012)
1 more