› 3vtv

X-ray diffraction
1.7Å resolution

Crystal structure of Optineurin LIR-fused human LC3B_2-119

Released:
DOI: 10.2210/pdb3vtv/pdb
PDB entry 3vtv coloured by chain, front view.
PDB entry 3vtv coloured by chain, side view.
PDB entry 3vtv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for phosphorylation-triggered autophagic clearance of Salmonella.
Rogov VV, Suzuki H, Fiskin E, Wild P, Kniss A, Rozenknop A, Kato R, Kawasaki M, McEwan DG, Löhr F, Güntert P, Dikic I, Wakatsuki S, Dötsch V
Biochem J 454 459-66 (2013)
PMID: 23805866

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Microtubule-associated proteins 1A/1B light chain 3B; Optineurin Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 15.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96CV9 (Residues: 170-181; Coverage: 2%)
  • Canonical: Q9GZQ8 (Residues: 2-119; Coverage: 94%)
Gene names: FIP2, GLC1E, HIP7, HYPL, MAP1ALC3, MAP1LC3B, NRP, OPTN
Sequence domains: Autophagy protein Atg8 ubiquitin like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 35.2Å b: 53Å c: 60.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.252
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Cargo recognition and trafficking in selective autophagy.
Stolz et al. (2014)
18 more

3 mentions without citation

Structure biology of selective autophagy receptors.
Kim et al. (2016)
2 more