› 3vu0

X-ray diffraction
1.94Å resolution

Crystal structure of the C-terminal globular domain of oligosaccharyltransferase (AfAglB-S2, AF_0040, O30195_ARCFU) from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb3vu0/pdb
PDB entry 3vu0 coloured by chain, front view.
PDB entry 3vu0 coloured by chain, side view.
PDB entry 3vu0 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Crystallographic and NMR evidence for flexibility in oligosaccharyltransferases and its catalytic significance.
Nyirenda J, Matsumoto S, Saitoh T, Maita N, Noda NN, Inagaki F, Kohda D
Structure 21 32-41 (2013)
PMID: 23177926

Function and Biology Details

Reaction catalysed: 
An archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dolichyl-phosphooligosaccharide-protein glycotransferase 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 162 amino acids
Theoretical weight: 19.56 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O30195 (Residues: 433-593; Coverage: 27%)
Gene names: AF_0040, aglB2
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MES 3 x MES
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P31
Unit cell:
a: 111.208Å b: 111.208Å c: 36.709Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.188 0.219
Expression system: Escherichia coli

Quick links

Citations

3 review citations

N-linked glycosylation in Archaea: a structural, functional, and genetic analysis.
Jarrell et al. (2014)
2 more

2 mentions without citation

Structural Insight into the Mechanism of N-Linked Glycosylation by Oligosaccharyltransferase.
Mohanty et al. (2020)
1 more