› 3vu7

X-ray diffraction
2.8Å resolution

Crystal structure of REV1-REV7-REV3 ternary complex

Released:
DOI: 10.2210/pdb3vu7/pdb
PDB entry 3vu7 coloured by chain, front view.
PDB entry 3vu7 coloured by chain, side view.
PDB entry 3vu7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of recruitment of DNA polymerase ζ by interaction between REV1 and REV7 proteins.
Kikuchi S, Hara K, Shimizu T, Sato M, Hashimoto H
J Biol Chem 287 33847-52 (2012)
PMID: 22859296

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-130061 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA repair protein REV1 Chain: H
Molecule details ›
Chain: H
Length: 124 amino acids
Theoretical weight: 14.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBZ9 (Residues: 1140-1251; Coverage: 9%)
Gene names: REV1, REV1L
Sequence domains: DNA repair protein REV1 C-terminal domain
Structure domains: DNA repair protein Rev1, C-terminal domain
Mitotic spindle assembly checkpoint protein MAD2B Chain: C
Molecule details ›
Chain: C
Length: 227 amino acids
Theoretical weight: 26.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI95 (Residues: 1-211; Coverage: 100%)
Gene names: MAD2B, MAD2L2, REV7
Sequence domains: HORMA domain
Structure domains: HORMA domain
DNA polymerase zeta catalytic subunit Chain: Z
Molecule details ›
Chain: Z
Length: 52 amino acids
Theoretical weight: 5.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60673 (Residues: 1847-1898; Coverage: 2%)
Gene names: POLZ, REV3, REV3L

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P3121
Unit cell:
a: 75.07Å b: 75.07Å c: 123.307Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.239
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Translesion DNA polymerases in eukaryotes: what makes them tick?
Vaisman et al. (2017)
17 more

17 mentions without citation

An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η.
Yang W. (2014)
16 more