› 3vv4

X-ray diffraction
2Å resolution

Crystal structure of cyanobacteriochrome TePixJ GAF domain

Released:
DOI: 10.2210/pdb3vv4/pdb
PDB entry 3vv4 coloured by chain, front view.
PDB entry 3vv4 coloured by chain, side view.
PDB entry 3vv4 coloured by chain, top view.
Source organism: Thermosynechococcus vestitus BP-1
Primary publication:
Structures of cyanobacteriochromes from phototaxis regulators AnPixJ and TePixJ reveal general and specific photoconversion mechanism.
Narikawa R, Ishizuka T, Muraki N, Shiba T, Kurisu G, Ikeuchi M
Proc Natl Acad Sci U S A 110 918-23 (2013)
PMID: 23256156

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 196 amino acids
Theoretical weight: 22.29 KDa
Source organism: Thermosynechococcus vestitus BP-1
Expression system: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: Q8DLC7 (Residues: 430-591; Coverage: 17%)
Gene name: tll0569
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

2 bound ligands:
Ligand PVG 2 x PVG
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P65
Unit cell:
a: 72.868Å b: 72.868Å c: 166.667Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.186 0.223
Expression system: Synechocystis sp. PCC 6803

Quick links

Citations

6 review citations

Phytochromes: an atomic perspective on photoactivation and signaling.
Burgie et al. (2014)
5 more

6 mentions without citation

Photoreceptor engineering.
Ziegler et al. (2015)
5 more