› 3vvo

X-ray diffraction
2.5Å resolution

Crystal structure of MATE in the bent conformation

Released:
DOI: 10.2210/pdb3vvo/pdb
PDB entry 3vvo coloured by chain, front view.
PDB entry 3vvo coloured by chain, side view.
PDB entry 3vvo coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Structural basis for the drug extrusion mechanism by a MATE multidrug transporter.
Tanaka Y, Hipolito CJ, Maturana AD, Ito K, Kuroda T, Higuchi T, Katoh T, Kato HE, Hattori M, Kumazaki K, Tsukazaki T, Ishitani R, Suga H, Nureki O
Nature 496 247-51 (2013)
PMID: 23535598

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MATE family efflux transporter Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 49.28 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U2X0 (Residues: 1-461; Coverage: 100%)
Gene name: PF0708
Sequence domains: MatE

Ligands and Environments

1 bound ligand:
Ligand OLC 10 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 152.893Å b: 58.835Å c: 71.491Å
α: 90° β: 93.18° γ: 90°
R-values:
R R work R free
0.211 0.206 0.256
Expression system: Escherichia coli

Quick links

Citations

52 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
51 more

8 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
7 more