› 3vw5

X-ray diffraction
2.6Å resolution

Crystal structure of sugar epimerase from ruminal bacterium

Released:
DOI: 10.2210/pdb3vw5/pdb
PDB entry 3vw5 coloured by chain, front view.
PDB entry 3vw5 coloured by chain, side view.
PDB entry 3vw5 coloured by chain, top view.
Source organism: Ruminococcus albus
Primary publication:
Crystal structure of Ruminococcus albus cellobiose 2-epimerase: structural insights into epimerization of unmodified sugar.
Fujiwara T, Saburi W, Inoue S, Mori H, Matsui H, Tanaka I, Yao M
FEBS Lett 587 840-6 (2013)
PMID: 23462136

Function and Biology Details

Reaction catalysed: 
Cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellobiose 2-epimerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 389 amino acids
Theoretical weight: 45.36 KDa
Source organism: Ruminococcus albus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DKY4 (Residues: 1-389; Coverage: 100%)
Gene name: ce-ne1
Sequence domains: N-acylglucosamine 2-epimerase (GlcNAc 2-epimerase)
Structure domains: Glycosyltransferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 98.93Å b: 100.88Å c: 186Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.222
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Enzymatic production of lactulose and 1-lactulose: current state and perspectives.
Wang et al. (2013)
1 more

5 mentions without citation

Structural insights into the epimerization of β-1,4-linked oligosaccharides catalyzed by cellobiose 2-epimerase, the sole enzyme epimerizing non-anomeric hydroxyl groups of unmodified sugars.
Fujiwara et al. (2014)
4 more