› 3vyw

X-ray diffraction
2.49Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MnmC-like methyltransferase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 308 amino acids
Theoretical weight: 36.19 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67789 (Residues: 1-308; Coverage: 100%)
Gene name: aq_1980
Sequence domains: S-adenosyl-L-methionine-dependent methyltransferase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SAM 4 x SAM
Ligand BEN 2 x BEN
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 55.487Å b: 107.581Å c: 117.711Å
α: 90° β: 102.44° γ: 90°
R-values:
R R work R free
0.195 0.191 0.255
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Methylated nucleosides in tRNA and tRNA methyltransferases.
Hori H. (2014)
3 more

2 mentions without citation

Structural basis for hypermodification of the wobble uridine in tRNA by bifunctional enzyme MnmC.
Kim et al. (2013)
1 more